qiaamp dna blood mini kit protocol


The QIAamp DNA Blood Mini Kit (Blood and Body Fluid Spin Protocol) is used to provide a fast and easy method for purification of total genomic DNA for reliable real time, quantitative PCR (qPCR) reactions. Blood samples from Bulgarian cattle 140 L of each serum sample was used for RNA isolation with the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers protocol. For blood, we used two different extraction protocols. The PureLink Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

A comprehensive guide to optimized immunoassay protocols.

The present invention also relates to a use based on the protein mutant, especially in enrichment of a target DNA and construction of sequence libraries. The activity of the Proteinase K solution is QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. 5.000000 out of 5. Material and Methods. Preamplification Kits; REPLI-g; dPCR Assays Kits & Instruments.

ZERO BIAS - scores, article reviews, protocol conditions and more If the sample volume is less than 200 l, add the appropriate volume of PBS. In case of 1mL inputs, you can adjust the volume of the sample to The QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit are intended for general laboratory use. artus EBV TM PCR Kit 03/2007 3 Table of Contents 1. Qiagen dneasy blood tissue kit qiagen Dneasy Blood Tissue Kit Qiagen, supplied by Qiagen, used in various techniques. Purified DNA was quantified with a Qubit 2.0 fluorometer (Thermo Scientific, Wilmington, DE). Time/Run, Manual (Centrifugation or Vacuum) Processing, Spin Columns Format, Silica Technology, CE/IVD Kit contents: Qiagen QIAamp DSP DNA Blood Mini Kit, 50 preps, 200L Sample, 50 to 200L Elution Volume, Whole Blood Sample, 20 min. To prolong the lifetime of Proteinase K, (2011) Sterile conditions: 20C: The QIAamp UCP Mini Columns and all buffers within the QIAamp UCP Pathogen Mini Kit undergo DNA decontamination processes and are certified to I highly recommended for molecular biology lab use. " DNA extraction Protocol for iSWAB-DNA using QIAamp DNA Mini kit (or DNeasy Blood & Tissue) Kits QIAamp DNA Mini and Blood Mini kit (Cat No 51104) Protocol 1. We followed this up with a second protocol using 2 ml of whole blood and the QIAmp DNA Blood Midi kit (Qiagen).

Bioz Stars score: 99/100, based on 1 PubMed citations. Following this, the DBS was processed through the kit a second time (Ex 2, El 1 and Ex 2, El 2). GenScript Biotech Corporation. Check the "added" box on the lid and date the lid. The QIAamp spin procedure uses a special lysate buffering condition that allows optimal binding of the DNA to the QIAamp membrane upon centrifugation.

Procedure 1. As reference protocols, the QIAamp DNA Blood Mini kit was used for extraction of DNA and the QIAamp Viral RNA Mini kit was used for extraction of RNA from 200-l samples as recommended by the manufacturer. Parasite DNA was extracted using QIAamp DNA mini kit (Qiagen, GmbH, Germany) procedure. Buffer, serum, and whole-blood samples were spiked with Bacillus anthracis Sterne vegetative cells and Yersinia pestis, while water was spiked with B. anthracis Sterne spores. The integrity of DNA/RNA is generally affected by a multitude of these factors, generating a large diversity of sample quality and highly variable target amplification (2). The QIAamp DSP DNA Blood Mini procedures, which are designed for The proteinase K is stable for up to 1 year after delivery when stored at room temperature.

Genomic DNA was extracted using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. Add the solution from step 9 onto a QIAamp Mini column. A protocol for DNA barcoding based on COI, DNA extraction of crushed cysts is performed using a QIAamp DNA mini kit DNA extractions are performed using a Qiagen DNeasy Blood and Tissue Kit, following the manufacturers protocol for animal tissues with the following modifications. (2011) Unknown: 80C: Cryopulverized using a freezer mill (Spex Certiprep Ltd) QIAamp DNA blood Midi kit (Qiagen) with modifications: Figuero et al. Of the 200 P. falciparum DNA samples screened, sequencing for mutations in K13, pfmdr-2, fd, pfcrt, arps10 genes was successful The QIAamp DNA Mini Kit contains Proteinase K, which is the enzyme of choice for SDS-containing lysis buffers used in the Tissue Protocol, but which performs equally well in the Blood and Body Fluid Protocol. 51304), starting at step 1. The following kits consistently yielded DNA that produced a detectable signal from blood spiked with 5.5104 colony forming units per mL: the High Pure PCR Template Preparation, QIAamp DNA Mini, MagNA Pure Compact, and the QIAcube running the QIAamp DNA Mini and QIAamp DNA Blood Mini kits. This study evaluated the Qiagen QIAamp DNA Mini Kit and the Schleicher and Schuell IsoCode Stix DNA isolation device for isolating nucleic acid.

Purification steps Notes 1. The initial protocol was performed using 200 l of whole blood and the QIAmp DNA Blood Minikit.

the QIAamp DNA Blood Mini Kit. The QIAamp TM 96 DNA QIAcube HT Kit (Qiagen), Macherey-Nagel Pathogne NucleoMag (Fisher Scientific), Mag-Bind TM Viral DNA/RNA (Omega Biotek), Chemagic Viral DNA/RNA 300 kit H96 (PerkinElmer) and Bioextract TM Superball TM (Biosellal) resulted in the worst efficiencies, with less than 35% of the sample being detected. Mix thoroughly by vortexing. The probe sets, purchased through Integrated DNA Technologies, can be found in Supplementary Table 1 . QIAamp DSP DNA Blood Mini Kit. The approximate processing time for 24 samples is 100 min. Figure Legend Snippet: Control Chelex 100 resin-based protocol versus QIAamp DNA Blood Mini Kit for the extraction of gDNA from DBS. Nail DNA from nail clippings of twin A and neonatal dried blood spot DNA were extracted using the QIAamp DNA Micro kit (QIAGEN) according to the manufacturers instructions. The spin column greatly reduces time consumption and is easy to do.

QIAamp Viral RNA Mini Kit Qiagen RNA Extraction Protocol

The extracted nucleic acids were resuspended in a volume of 50 l of aqua bidest. Videos. Genomic DNA was extracted from 1 mL of whole blood (n = 5) or plasma (n = 92) using the Gentra PureGene Blood Kit (Gentra, Minneapolis, MN) and the QIAamp DNA Blood midi kit (Qiagen, Inc., Valencia, CA), respectively, following the manufacturer's instructions, and was reconstituted in a buffer containing 10 mmol/L Tris (pH 7.6) Check out videos that might be relevant for performing DNA isolation / purification Cells - primary cells lymphocytes using QIAamp DNA Blood Mini Kit from Qiagen. Blood and Tissue Products; Nucleic Acid Purification; Liquid Handling Workstations; Microscopes and Cell Imagers; Chromatography and Separation; Protein Purification and Sample Prep; For plasma isolation, the blood sample was first centrifuged at 1600 g for 10 min at 4 C to separate the plasma from peripheral blood cells. Herring sperm DNA was diluted to 2 g/mL with TE (10 mM Tris, 1 mM EDTA, pH.This process uses the Quant-iT PicoGreen dsDNA quantitation reagent to quantitate double-stranded DNA samples for the GoldenGate Genotyping Assay..Quant-iT PicoGreen dsDNA Assay Kit, 1ml kit $ 552. qRT-PCR analysis of the RNA viral genome or vector-derived Disclosed is a structure for detecting cell-free DNA using a conductive polymer and the use thereof. Storage..6

As reference protocols, the QIAamp DNA Blood Mini kit was used for extraction of DNA and the QIAamp Viral RNA Mini kit was used for extraction of RNA from 200-l samples as recommended by the manufacturer. The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, including muscle, liver, heart, brain, bone marrow and other tissues, swabs (buccal, eye, nasal, pharyngeal and others), CSF, blood, body fluids and washed cells from urine. The nucleated cellular fraction or buffy coat remaining after removal of the plasma can be processed for gDNA automated with the QIAsymphony DSP DNA Mini and Midi Kits, or manually with the QIAamp DSP DNA Blood Mini Kit. Besides that shown in Table 2, their protocols are much more tedious and require large amounts of starting material for making a sequencing library (500 pg1 g as shown in page 4 of NEBNext UltraII DNA Library Prep Kit for Illumina, or > 100200 ng input gDNA as shown in page 5 of TruSeq Nano DNA Library Prep Reference Guide). Download manufacturer protocol. dPCR Instruments; dPCR Kits; dPCR Assays; dPCR Accessories; Custom LNA Oligonucleotides; Next-Generation Sequencing. The proteinase K is stable for up to 1 year after delivery when stored at room temperatur e. To prolong the lifetime of Proteinase K, storage at

This study aimed to compare the quantity and quality of human DNA extracted from saliva that was fresh or frozen for three, six and twelve months using five different DNA extraction protocols: protocol 1 Oragene commercial kit, protocol 2 QIAamp DNA mini kit, protocol 3 DNA extraction using ammonium acetate, protocol 4 Instagene Matrix and Member since: 2011. None of the studies used the exact same PCR protocol and DNA extraction kit. The QIAamp UCP Pathogen Mini Kit provides fast and easy methods for purification of microbial DNA from a variety of sample materials such as whole blood, swabs, biological fluids, and cultures. Read independent reviews on QIAamp DNA Mini QIAcube Kit (240) from QIAGEN on SelectScience .

What protocol in the QIAamp blood mini kit do you use to extract DNA from isolated PBMCs? QIAamp Viral RNA Kits simplify viral RNA purification from cell-free body fluids with fast spin-column, vacuum and plate centrifugation procedures. QIAamp DNA mini kit (Qiagen, Valencia, Spain) Delbosc et al. 51304), starting at step 1. The brief version of the procedure is below for reference. The kits are designed for a range of sample sizes from 200 l up to 10 ml fresh or frozen human whole blood. The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery of DNA; the QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table DNA yields from human whole blood with different cell densities). Qiaamp Dna Mini Kit Blood, supplied by Qiagen, used in various techniques. Organization: National Institute of Mental Health and Neurosciences; Ease of Whole Genome Sequencing; Whole Exome Sequencing; Targeted DNA Panels; Single Cell DNA; Custom DNA Panels; Whole Genome; RNA Sequencing. Browse by Field. The TruSight Myeloid Sequencing Panel (Illumina, San Diego, USA) was used, starting from 50 ng of genomic DNA and according to the manufacturers instructions. The QIAamp DNA Mini kit provides a fast and easy method for the purification of DNA from lymphocytes for reliable PCR and Southern Blotting. of HBV-positive serum using the QIAamp DNA Blood Mini Kit (QIAamp) or an SDSphenol method (SDS/phenol) and analyzed by agarose gel electrophoresis. qPCR and qRT-PCR. The DNA from the grains of two maize hybrids, M10 and M321, was extracted using extraction methods DNeasy Qiagen Plant Mini Kit, CTAB-method (with/without 1% PVP) and modified Mericon extraction. Place the QIAamp Mini spin column in a new 1.5 ml microcentrifuge tube (not provided), add 200 l Buffer AE or distilled water and incubate at room temperature for 1 min. Changes to the protocol: In step 11, elute samples in 50 L Buffer AE. In accordance with the manufacturers instructions, DNA was purified using the QIAamp Blood DNA Mini Kit (Qiagen, UK) by fast spin-column procedures. ciparum parasite DNA samples were screened. of pathogenic bacteria. No. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a Question. What is the best kit or protocol for DNA genome extraction from blood sample?

Add 200 l Buffer AL. The QIAamp DSP DNA Blood Mini Kit is intended for in vitro diagnostic use. 2. Contents..5 2. The present invention relates to a mutant of Argonaute protein lacking a DNA cleavage activity but having a DNA binding activity, wherein the mutation of the mutant is located in a PIWI domain. Read PDF Qiaamp Dna Mini Kit And Qiaamp Dna Blood Mini Kit Handbook Pyrosequencing[Registered] Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing[Registered] technology, all written by world-renowned experts in the field. when transferring the supernatant. Carefully open the QIAamp Mini spin column and add 200 L Buffer AEor distilled water. Great instrument, easy to use it! The purified DNA is free of proteins, nucleases and other contaminants and inhibitors. Cell-free DNA from 900 L of maternal plasma was extracted using the QIAamp DNA Blood MiniKit (Qiagen, Hilden, Germany) following the manufacturers protocol. 3. no. At Step 2 of the QIAGEN QIAamp DNA blood mini kit protocol (QIAamp DNA mini and blood mini handbook 06/2012, Protocol: DNA purifi cation from blood or body fl uids), add Decant from the buffer stock bottles into the matching QIAcube reagent bottles. Genomic DNA of pri-mary tumors was extracted from formalin-fixed paraffin-embedded QIAamp DNA Blood Mini Kit: Quick-DNA Miniprep Plus: Product No. Description QIAamp DNA Blood Mini Kit provides silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. 4. 2.3. GenScript Biotech Corporation is the world leading science serving platform by providing reliable, high quality and innovative reagents and instruments with superior customer service to enable customer successes across a wide variety of existing and emerging life science research and development areas. QIAamp DNA mini kit (QIAGEN Cat. Close the cap and centrifuge at 3,750 rpm for 30 seconds. Follow the DNA purification from blood or body fluids (spin protocol) procedure in the QIAamp DNA mini kit handbook (QIAGEN Cat. Incubate at room temperature (1525C) for 1 minute, and then centrifuge at 6000 x g (8000 rpm) for 1 minute. Item QIAamp DNA Blood Mini Kit (250) Company QIAGEN - Sample to Insight; Catalog Number 51106; This product is no longer available on Biocompare. QIAamp DNA Mini Kits contain ready-to-use proteinase K solution, which is dissolved in a specially formulated storage buffer. The PCR products were sequenced using Sanger dideoxy sequencing method. M: 100 bp ladder. 3. Principle and procedure QIAamp DNA Mini and QIAamp DNA Blood Mini Kits are designed for rapid purification of an average of 6 g of total DNA (e.g., genomic, viral, mitochondrial) from 200 l of whole human blood, and up to 50 g of DNA from 200 l of buffy coat, 5 x 10 6 lymphocytes, or cultured cells that have a normal set of chromosomes. 3. To characterize the immune cells in HCC, we applied scRNA-seq methods to study CD45 + cells isolated from tumors and four immune-relevant sites (adjacent liver, hepatic LNs, blood, and ascites) of 16 treatment-naive liver cancer patients (Figures S1AS1C; Tables S1 and S2).For patient DSN09, both 10x Genomics and SMART-seq2 methods were applied in parallel, Bioz Stars score: 97/100, based on 1 PubMed citations. QIAamp DNA Mini Kit (250) from QIAGEN - Sample to Insight. Product Use Limitations The QIAamp RNA Blood Mini Kit is intended for molecular biology applications. The dissected tissues were subjected to DNA extraction and purification using a QIAamp DNA FFPE Tissue kit (Qiagen Gmbh, Hilden, Germany). Publication protocol Pharmacogenetic analysis. Place the QIAamp Mini spin column in a clean 1.5 mL microcentrifuge tube (not provided), and discard the collection tube containing the filtrate. Following this, the DBS was processed through the kit a second time (Ex 2, El 1 and Ex 2, El 2).

Other contaminants are removed by salt precipitation. Dove medical press limited, fill a single gene, and proteins that there is also covered below to find, viral and assay tube. Techniques: Amplification, Serial Dilution, Nested PCR, Negative Control. Protocol: Isolation of genomic DNA Mini kit Purpose: This protocol is used to extract gDNA from a frozen cell pellet of less than 5e6 cells using the QIAamp DNA Blood Mini Kit (Qiagen Cat# 51104). Stephanie De Souza, University at Albany - SUNY QIAamp DNA Blood Mini Kit (250) Venkataram Shivakumar; Status: Reviewer . Pipette 200-300l from iSWAB collected sample into a clean labeled 1.5ml microcentrifuge tube. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. We have also compared 2 Qiagen kits, this one and the "DNeasy Blood & Great product! Figure Lengend Snippet: Control Chelex 100 resin-based protocol versus QIAamp DNA Blood Mini Kit for the extraction of gDNA from DBS. 2. 10.Optional: Repeat step 10 for increased DNA yield with a further 200 l Buffer AE or distilled water. Alcohol is added and lysates loaded onto the QIAamp spin column. Quick Start Protocol: QIAamp Blood Mini Kit QIAamp DNA Mini and blood mini handbook, Th ird Edition, June 2012; Protocol: DNA purifi cation from blood or body fl uids: Page 26. 2. QIAamp Blood Mini Kit, HB-2529. This comprehensive volume enables quick reference by collecting the primary

Genomic DNA was isolated from embryos using a QIAamp DNA Mini Kit (Qiagen, 51304). The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 l. Free fulltext PDF articles from hundreds of disciplines, all in one place Biocompare is the leading resource for up-to-date product information, product reviews, and

QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit 02/200341 Dried Blood Spot Protocol Dried Blood Spot Protocol Notes: This protocol is suitable for blood, both untreated and treated with anticoagulants, which has been spotted and dried on filter paper (Schleicher and Schuell 903). Quality Management System, each lot of QIAamp DSP DNA Blood Mini Kit is Clean bench area.

Cap the bottles and shake well to mix. Load the reagent bottles into the reagents rack in the robot according to the positions shown in Figure 1.

Blood Mini Handbook For DNA purification from whole blood plasma serum buffy coat lymphocytes dried blood spots (QIAamp DNA Mini Kit only) body fluids cultured cells Isolate genomic dna extraction is the kit for dna mini spin columns are targeted to the kit protocol from qiagen blood dna extraction from unpurified plasma from proteins. Procedure 1. Review date: 19 May 2014 | QIAamp DNA Blood Mini Kit (50) "Easiest protocol I've ever seen." QIAamp DNA Blood Mini Kit was performed with a first (Ex 1, El 1) and second elution (Ex 1, El 2). Commercially available kits for manual and automatic extraction of nucleic acids from formalin-fixed, paraffin-embedded (FFPE) tissues. Genomic DNA from 3D7 and 7G8 strains was extracted from cultures grown at WRAIR using QIAamp DNA Blood Mini Kit. This product is not intended for the diagnosis, prevention, or treatment of a disease. Download the product protocol from Qiagen for QIAamp DNA Blood Mini Kit below. By using the nanostructure, cell-free DNA including circulating tumor DNA, can be effectively detected and isolated. QIAamp DNA Blood Midi/Maxi Handbook Print Bookmark Share For large-scale genomic and viral DNA purification from whole blood, plasma, serum, body fluids, lymphocytes pdf 463KB English Format File size Language Download Get Adobe Reader QIAamp DNA Blood Mini Kit was performed with a first (Ex 1, El 1) and second elution (Ex 1, El 2). 21 answers. It is also recommended that a DNA sample derived from a healthy blood donor is included in every Pyrosequencing run for comparison. Pipet 20 l QIAGEN Protease into a 1.5 ml microcentrifuge tube. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. It is easy to use, rapid and very good quantity DNA isolation from blood or body fluids with almost nil contamination. qPCR and qRT-PCR have become mainstream life science technology for the quantitation of nucleic acids.22 The focus of this paper is to mainly describe qPCR analysis of DNA vectors as an example to discuss the approach of method development, validation, and sample analysis. 2. Summary and Explanation The QIAamp DSP DNA Blood Mini Kit uses well-established technology to provide a fast and easy way to isolate and purify genomic DNA from 200 l whole blood. Centrifuge your isolated PBMCs and then discard the supernatant. Negative controls, consisting of water (guaranteed DNA and RNA free; Sigma), were processed after every four clinical samples. The kits are designed for a range of sample sizes from 200 l up to 10 ml fresh or frozen human whole blood. All Methods. 2.7 | DNA extraction from leukocyte and formalin- fixed paraffin-embedded tissues The CD45-positive fractions isolated with autoMACS were analyzed as paired normal leukocytes. 51304) and required reagents for DNA purification from blood or body fluids (spin protocol) procedure. Add 200 l sample. Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Optimized components and protocols The PureLink Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). QIAampRNA Blood Mini Kits should be stored dry, at room temperature (1525C) and are stable for at least 9 months under these conditions. QIAamp DNA Mini Kits contain ready-to-use proteinase K solution, which is dissolved in a specially formulated storage buffer. Except for the QIAamp MinElute ccfDNA Mini Kit, all extraction methods yielded significantly less total ccfDNA compared with the QIAamp Circulating Nucleic Acid Kit method in multilevel linear regression analysis (Supplemental Table 2B). Add 200 microlitres of lysis solution and 20 microlitres of Proteinase K, briefly vortex, and incubate at with the QIAsymphony PAXgene Blood ccfDNA Kit, or manually with the QIAamp DSP Circulating Nucleic Acid Kit. For purification of genomic DNA from human whole blood for in vitro diagnostic use. The protocol was performed by using 300 l plasma and eluting the DNA with 30 l of elution buffer (AE) in 2 steps, with 20 l and 10 l AE and incubation time of 5 min each. Blood Mini Kit This protocol uses the QIAamp DNA Blood Mini Kit to purify genomic DNA from the buffy coat fraction Protocol) in the QIAamp DNA Mini and Blood Mini Handbook, starting with step 3, where 200 l Buffer AL are added to the sample. The QIAamp DNA Stool Mini Kit simplifies isolation of DNA from stool with a fast spin-column procedure (see flowchart " Procedure ").

ZERO BIAS - scores, article reviews, protocol conditions and more. No. In our lab we use 'QIAamp DNA Blood Mini Kit' from Qiagen; this kit provides quite good DNA yield. Video tutorials of the Split procedure:1. More particularly, a nanostructure composed of a surface-modified conductive polymer for isolating and detecting cell-free DNA is disclosed. Paperity: the 1st multidisciplinary aggregator of Open Access journals & papers.

DNA Sequencing. BioSprint 96 DNA Blood Kit (384) 5.000000 out of 5. Cannabis Testing; Clinical Diagnostics; QIAamp DSP DNA Blood Mini Kit. The QIAamp DNA FFPE Tissue Kit uses well-established QIAamp MinElute technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes. Home > Search Results > Qiagen > qiaamp dna mini kit blood. Read More.

Centrifuge at 6000 x g (8000 rpm) for 1 min to elute the DNA. Be useful as kit protocol, blood mini kit and protocols for each of interest for up your own lysate. Tissue should be no larger than a grain of rice. PyroMark Q24 run (Protocol 5) Analysis of PyroMark Q24 run Report Controls Methylated control DNA is included in the kit as a positive control for PCR and sequencing reactions. Follow the DNA purification from blood or body fluids (spin protocol) procedure in the QIAamp DNA mini kit handbook (QIAGEN Cat. Procedure 1. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. In the Tilburg laboratory, the Qiagen columns (QIAamp DNA mini kit; Qiagen, Hilden, Germany) have been used for the extraction of DNA from sputum samples or BAL samples, to be tested by the Legionella PCR/probe assay. Preheat a water bath or heating block to 56C. Salt and pH conditions RNA was eluted in 60 L of Buffer AVE and stored at 80 C until further use. Genomic DNA was then extracted using a QIAamp DNA Mini Kit (QIAGEN). The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, including muscle, liver, heart, brain, bone marrow and other tissues, swabs (buccal, eye, nasal, pharyngeal and others), CSF, blood, body fluids and washed cells from urine.